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His lab analyzes interactions between these molecules using an impressive array of high-throughput technologies, capable of carrying out the molecular equivalent of speed dating. Tens of thousands of molecules can be rapidly screened against as many potential partners. Protein-protein binding is central to all cellular processes; finding new associations and demonstrating direct interactions are crucial pieces of evidence in determining molecular functions.

The method uses yeast cells containing baits — binding domains from proteins that have been attached to a reporter system. The platform has added two enhancements of the traditional Y2H system: MYTH, which enables scientists to study proteins bound to membranes or molecules they interact with, and CytoY2H, which detects interactions between transcriptionally active proteins.

The lab has developed another technology called the DULIP dual luminescence-based co-immunoprecipitation assay, which is based on similar principles but operates in mammalian cells and can be used to map their interactomes. Robotics innovations developed by the group have turned the assay into a high-throughput method with advantages over traditional co-immunoprecipitation and Western blotting assays, in terms of speed and sensitivity.

While defects in single molecules may trigger pathological processes, many more proteins are usually involved in bringing diseases to their full-blown forms. Clarifying details of their interactions has helped Erich and his colleagues identify weak points in the structures of fibers and aggregations that make good targets for inhibitors. The X and Y chromosomes, also known as the sex chromosomes, determine the biological sex of humans.

Yes, a lot of what they say about proteins is true: they are often promiscuous, and have trouble maintaining long-term attachments In an experiment or model, two molecules may flirt with each other and look like a couple, but is their interaction really intimate and direct? Photo: Wanker lab, MDC.

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