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His lab analyzes interactions between these molecules using an impressive array of high-throughput technologies, capable of carrying out the molecular equivalent of speed dating. Tens of thousands of molecules can be rapidly screened against as many potential partners. Protein-protein binding is central to all cellular processes; finding new associations and demonstrating direct interactions are crucial pieces of evidence in determining molecular functions.
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The lab has developed another technology called the DULIP dual luminescence-based co-immunoprecipitation assay, which is based on similar principles but operates in mammalian cells and can be used to map their interactomes. Robotics innovations developed by the group have turned the assay into a high-throughput method with advantages over traditional co-immunoprecipitation and Western blotting assays, in terms of speed and sensitivity.
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